Present investigation is focused on the “Preclinical evaluation of antidepressant activity of ethanolic extract Tribulus terrestris fruits and n- butanol fractions of ethanolic extract of Tribulus terrestri fruits (NETT) were evaluated for antidepressant activity using in laboratory animals using different preclinical models of antidepressant activity such as forced swim test, tail suspension test and spontaneous locomotor activity. The preliminary phytochemical analysis revealed the phytoconstituents present in the extracts. Ethanolic extract of Tribulus terrestris fruits (ETT) showed the presence of steroids, saponins, tannins, terpenoids, flavonoids, alkaloids. n-butanol fractions of ethanolic extract of Tribulus terrestris fruits (NETT) were subjected to the High-Performance Thin Layer Chromatography (HPTLC) studies Ethanolic extract of Tribulus terrestri fruits (NETT) and their fractions showed potent antidepressant activity. The multistep putative action of these plants may be attributed to the prominent phytoconstituents namely 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydrochromen-4-one from n-butanol fractions of ethanolic extract of Tribulus terrestris fruits (NETT).  As a result of these studies two different phytoconstituents were isolated i.e., 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydrochromen-4-one from n-butanol fractions of ethanolic extract of Tribulus terrestris fruits (NETT). The isolation of these phytoconstituents is an important concluding result of the study and cardioprotective activity of the extracts may be attributed to these Phytoconstituents